Breast cancer diagnostics with gold nanoparticles
The early diagnosis and treatment of breast cancer nodes is an important first step in the healing regime for this disease. A current study targets breast cancer cells with specially treated gold nanoparticles and using electrical impedance measurements in a specially designed chamber. Impedance (a form of electrical resistance) readings can be taken for cell suspensions using breast cancer and skin cells.
Technically, Herceptin-conjugated gold nanoparticle in cells can be used to detect and record the change in impedance value when tested in a specially designed container. The electrical properties of single cells, including whole cell conductivity, membrane capacitance, relaxation frequency, etc. can be derived for each cell line. The surface properties of the gold nanoparticles and drug loaded nanoparticles can be characterized by Fourier transform infrared (FT-IR) spectroscopy, ultraviolet and visual band spectrometry, and dynamic light scattering (DLS). To evaluate the electrical properties and cytotoxicity of the gold nanoparticles and Herceptin conjugated particles, human breast cancer cells have to be cultured in the presence of drug conjugated nanoparticles. The intracellular uptake of Gold nanoparticle-Herceptin to the cells can be observed by impedance measurements and confocal laser scanning microscopy. The outcome of this research work will find application in clinical trials of cancer treatment drugs.Share on